Yoichi Araki Ph.D.

 

Present address
Yochi Araki Ph.D.
Department of Neuroscience
Johns Hopkins University, School of Medicine
725 North Wolfe St. PCTB1001
Baltimore 21205 USA
Tel +1-410-955-4051
Fax +1-410-955-0877
e-mail yoichi.araki@jhmi.edu

Hometown: Yokohama, Japan
Status: Post-Doc Fellow (Dr. Richard Huganir's Lab) in Johns Hopkins University, School of Medicine
Google reader: http://www.google.com/reader/shared/08768153380521977591

Education
2007.4-present Johns Hopkins University, School of Medicine, Post-Doc Fellow (Dr. Richard Huganir Lab) [Member List]
2005.4- 2008.3 JSPS Fellow (Fellowships for Young Scientists) in Hokkaido Universiy, Graduate school of Pharmaceutical Sciences (Dr. Toshiharu Suzuki Lab)
2005.3 Ph.D. degree(Pharmaceutical Sciences), University of Tokyo (Mentor: Dr. Toshiharu Suzuki.) (March 24, 2005)
2002.3 Master degree(Pharmaceutical Sciences), University of Tokyo (Mentor: Dr. Toshiharu Suzuki.) (March 29, 2002)
2000.3 Bachelor degree, University of Tokyo (Mentor: Dr. Yutaka Kirino, Dr. Toshiharu Suzuki & Dr. Susumu Tomita) (March 28, 2000)
*JSPS : JapanSociety for Promotion Science

Birth
April 1, 1976 Yokohama, JAPAN

Scientific Skills
*Basic Molecular biology tecniques (including site-directed mutagenesis, PCR, RT-PCR, DNA sequencing, In vitro translation, RNA isolation)
*Basic Cellular biology tecniques (including density gradient fractionation(Organelle isolation), immunostaining, immunohistochemistry, primary cultured neuron)
*Live cell imaging (multi-color Total Internal Reflection Microscopy at Video Frame Rate, FLIP, FRAP)
*Cell culture, transfection(including primary cultured neuron using calcium-phosphate method), ELISA(Abeta-assay), RIA
*Low density hippocampal culture (gbankerh culture) and transfection
*High efficency transfection of Genes into Primary cultured neurons by Lenti- or Sindbis virus
*Gene cloning and expression
*siRNA expreriments
*Western-blotting, Far-Western blooting
*Protein isolation and purification
*In vivo experiments (operation of small animals)
*Antibody production(Rabbit polyclonal, mouse monoclonal antibodies, efficient sequence prediction especially for peptide antigen)
*Yeast two hybrid screening (GAL4 system)
*MALDI-TOF MS including finger printing to identify protein of interest (with 2D-electrophoresis)
*MALDI TOF MS/MS (determine amino acid sequence, and Phosphorylation site)
*In vitro direct binding assay (including Surface Plasmon Analysis(BIACORE), Scatchard Plot Analysis)
*Isotope experiments (Pulse-chase study using 35S, Phophorylation analysis using 32P)

Publications . . [Search PubMed]

**Peer-reviewd journals
4. Akio Sumioka, Yuhki Saito, Megumi Sakuma, Yoichi Araki, Tohru Yamamoto and Toshiharu Suzuki
The X11L/X11beta/MINT2 and X11L2/X11gamma/MINT3 scaffold proteins shuttle between the nucleus and cytoplasm
Exp Cell Res., In press (2008) [PubMed]

3. Yoichi Araki, Takanori Kawano, Hidenori Taru, Yuhki Saito, Sachiyo Wada, Kanako Miyamoto, Hisako Kobayashi, Hiroyuki O. Ishikawa, Yu Ohsugi, Tohru Yamamoto, Kenji Matsuno, Masataka Kinjo, and Toshiharu Suzuki
The novel cargo Alcadein induces vesicle association of kinesin-1 motor components and activates axonal transport
EMBO J. 26, 1475-1486 (2007) [PubMed]

2. Yoichi Araki, Naomi Miyagi, Naoko Kato, Tomohiro Yoshida, Sachiyo Wada, Masaki Nishimura, Hiroto Komano, Tohru Yamamoto, Bart De Strooper, Kazuo Yamamoto, Toshiharu Suzuki
Coordinated metabolism of Alcadein and amyloid beta-protein precursor regulates FE65-dependent gene transactivation
J. Biol. Chem. 279, 24343-24354 (2004) [PubMed]  [Alzforum]

1. Yoichi Araki, Susumu Tomita, Haruyasu Yamaguchi, Naomi Miyagi, Akio Sumioka, Yutaka Kirino, Toshiharu Suzuki
Novel Cadherin-related membrane proteins, Alcadeins, enhance the X11-like protein-mediated stabilization of amyloid beta-precursor metabolism
J. Biol. Chem. 278, 49448-49458 (2003) [PubMed]

**Reveiw
1. Toshiharu Suzuki, Yoichi Araki, Tohru Yamamoto, Tadashi Nakaya
Traffcking of Alzheimerfs Disease- Related Membrane Protein and Its Participation in Disease Pathogenesis
J Biochem 139, 949-955 (2006) [PubMed]

**Review (Japanese)
1. Yoichi Araki
Current Topics
Experimental Medicine (JP) 26(1:January), 65-68 (2008) [link]

**Book chapters (Japanese)
2.@Toshiharu Suzuki, Yoichi Araki, Megumi Sakuma

Single molecule observation of Alzheimersf-related proteins in living cells

Bio and Nano United-II (New Areas of Biology Series) (Japanese)
ISBN978-4-8329-8178-2 2007.3.30 / Chaptor. 10 (2007) [link]


1. Yoichi Araki, Toshiharu Suzuki

Protein misfolding and Neurogegenerative Diseases

Protein Science Illustrated (Japanese) Chaptor. 6-1  p286-294 (2005) [link]

**Symposium
2. Yoichi Araki
Total Internal Reflection Microscopy (TIRFM) : Next generation of Live cell imaging, and their abilities to visualize vesicle traffcking in neurons
8th Cell Biology Workshop (21th century COE program) Invited Lecturer
January 21-26, 2007 Hokkaido, JAPAN

1. Yoichi Araki, Toshiharu Suzuki
Cell biology of Alzheimerfs Disease : Physiological functions of APP and Alcadein
9th Pharma Science Forum (Hokkaido University)
June 18, 2005 @Hokkaido, JAPAN

**Presentation of International Conference
14. Keiko Furukori, Yoichi Araki, Hidenori Taru, Tohru Yamamoto, Roger J. Davis, Toshiharu Suzuki
Analysis of axonal transport of APP in JIP1 gene-deficient mice
11th International Conference of Alzheimerfs Disease and Related Disorders,
July 27, 2008 [P1-452] Chicago, USA

13. Saori Hata, Yoichi Araki, Katsuya Urakami, Hiroyasu Akatsu, Masaki Nishimura, Sam Gandy, Tadashi Nakaya, Toshiharu Suzuki
Analysis of b-Alc; Alcadein processing yields fragment homologous to APP P3 peptide
11th International Conference of Alzheimerfs Disease and Related Disorders,
July 30, 2008 [P3-072] Chicago, USA

12. Mayu Akiyama, Megumi Sakuma, Yoichi Araki, Sayaka Fujishige, Tadashi Nakaya, Toshiharu Suzuki
Mechanism of a tripartite complex formation composed of APP, X11L, and Alcadein
37th Annual Meeting of Society for Neuroscience (Neuroscience2007)
November 7, 2007 [884.17] San Diego, USA

11. Saori Hata, Yoichi Araki, Toshiharu Suzuki
Metabolism of Alcadein and its product b-Alca :Novel diagnostic marker for Alzheimerfs disease
gNeurodegenerative disease: Biology & Therapeuticsh in Cold Spring Harbor Laboratory meeting 2006
November 30-December 3, 2006 CSHL, NewYork, USA

10. Toshiharu Suzuki, Yoichi Araki, Takanori Kawano, Emi Tanaka, Takako Ishikawa, Tadashi Nakaya, Yu Ohsugi, Tohru Yamamoto, Masataka Kinjo
Trafficking and metabolism of APP and Alcadein
36th Annual Meeting of Society for Neuroscience (Neuroscience2006)
October 16, 2006 [672.25] Atlanta, USA

9. Yoichi Araki, Takanori Kawano, Toshiharu Suzuki
Alcadein is a novel cargo receptor for Kinesin-I, and JIP1 selects Kinesin binding prefernce whether Alcadein or APP affects Ab generation
10th International Conference of Alzheimerfs Disease and Related Disorders
July 19, 2006 [P4-042] Madrid, SPAIN

8. Saori Hata, Yoichi Araki, Hiroyasu Akatsu, Katsuya Urakami, Masaki Nishimura, Tadashi Nakaya, Toshiharu Suzuki
b-Alca, metabolic product of Alcadein, as a novel diagnostic marker in CSF of Alzheimerfs Disease
10th International Conference of Alzheimerfs Disease and Related Disorders,
July 19, 2006 [P4-020] Madrid, SPAIN

7. Takanori Kawano, Yoichi Araki, Tadashi Nakaya, Kenji Matsuno, Toshiharu Suzuki
Analysis of Alcadein isoform function in vesicular transport
10th International Conference of Alzheimerfs Disease and Related Disorders,
July 19, 2006 [P1-376] Madrid, SPAIN

6. Takanori Kawano, Yoichi Araki, Tadashi Nakaya, Kenji Matsuno, and Toshiharu Suzuki
Function of Alcadein in vesicular transport
20th IUBMB International Congress of Biochemistry and Molecular Biology and 11thFAOBMB Congress(2006)
June18-23 2006 Kyoto, JAPAN

5. Yoichi Araki, Takanori Kawano, Shiho Naito, Hidenori Taru, Tohru Yamamoto, Toshiharu Suzuki
Alcadein, a Presenilin/gamma-secretase substrate is a novel cargo receptor for Kinesin-I
35th Annual Meeting of Society for Neuroscience (Neuroscience2005)
November 14, 2005 [543.2] Washigton DC, USA

4. Yoichi Araki, Naomi Mkiyagi, Tomohiro Yoshida, Sachiyo Wada, Tohru Yamamoto, Toshiharu Suzuki
Coordinated mebolism of Alcadein and amyloid ƒŔ-protein precursor regulates FE65-dependent gene transactivation
34th Annual Meeting of Society for Neuroscience (Neuroscience2004)
October 23, 2004 [91.9] San Diego, USA

3. Yoichi Araki, Naomi Miyagi, Naoko Kato, Tomohiro Yoshida, Toshiharu Suzuki
Presenilin-dependent gamma-secretase cleavage of Alcadein and Amyloid precursor protein; their coodinative metabolism and cooperative regulation on FE65-dependent gene transactivation
9th International Confernce of Alzheimerfs Disease and Related Disorders
July 21, 2004 [P4.299] Philadelphia, USA

2. Naomi Miyagi, Megumi Sakuma, Yoichi Araki, Toshiharu Suzuki
X11L and Alcadein form a Tripartite complex with APP and supress the gamma-cleavage of CTFbeta
9th International Confernce of Alzheimerfs Disease and Related Disorders
July 18, 2004 [P1.225] Philadelphia, USA

1. Yoichi Araki, Haruyasu Yamaguchi, Susumu Tomita, Toshiharu Suzuki
Coupling of APP with novel membrane proteins through neuron-specific adaptor protein, X11-like
8th International Confernce of Alzheimerfs Disease and Related Disorders
July 22, 2002 [707] Stockhorm, SWEDEN
*-oral or symposium  presenter


**Presentation of Domestic (Japan) Conference
22. Arasaki M., Nozawa K., Furukori K., Araki Y., Yamamoto T., Kinjo M., Suzuki T
Molecular mechanisms of JIP-1 as linker between APP and Kinesin-1 and
Regulation of APP trafficking in neurons
31th Annual Meeting of Japan Molecular Biology Society/81th Annual meeting of Japan Biochemical Society (BMB2008)
December 9-12, 2008 [4P-0559] Kobe, JAPAN

21. Keiko Furukori., Shiho Naito., Yoichi Araki., Tohru Yamamoto.,  Toshiharu Suzuki
Mechanism of kinesin-1 motor connection of APP via JIP-1b
30th Annual Meeting of Japan Molecular Biology Society/80th Annual meeting of Japan Biochemical Society (BMB2007)
December 11, 2007 Yokohama, JAPAN

20. Hisako Kobayashi, Hiroyuki Ishikawa, Yoichi Araki, Toshiharu Suzuki, Kenji Matsuno
Drosophila Ortholog of Alzheimer's reated gene, alcadein, functions in axonal vesicular trafficking
29th Annual Meeting of Japan Molecular Biology Society
December 6-9, 2006 Yokohama, JAPAN

19. Chie Mizumaru, Yoichi Araki, Tadashi Nakaya, Toshiharu Suzuki
Requlation of gamma-secretase activity by AID
78th Annual meeting of Japan Biochemical Society
October 22, 2005 Kobe, JAPAN

18. Takanori Kawano, Yoichi Araki, Tadashi Nakaya, Kenji Matsuno, Toshiharu Suzuki
Identification of Kinesin light chain binding sites of Alcadein, novel cargo receptor
78th Annual meeting of Japan Biochemical Society
October 22, 2005 [4P-313] Kobe, JAPAN

17. Toshiharu Suzuki, Yoichi Araki, Eiko Kawaguchi, Katsuya Urakami, Masaki Nishimura, Sayaka Fujishighe, Takao Ishikawa, Tadashi Nakaya
ƒŔ-Alca, metabolic product of Alcadeina is a potential marker for Alzheimerfs Disease
24th Annual meeting of Japan Dementia Socirty
September 30-October 1, (Japan Society of Dementia Award), 2005 Osaka, JAPAN

16. Yoichi Araki*, Hidenori Taru, Toshiharu Suzuki
Roles of JIP1 and X11L on the metabolism and transport of APP and Alcadein
Neuroscience 2005 (28th Annual meeting of Jpapan society for Neuroscience)
July 27, 2005 [SY2-10-4] *Invited Lecturer. Yokohama, JAPAN

15. Yoichi Araki, Chie Mizumaru, Akio Sumioka, Megumi Sakuma, Katsuya Omori, Yuhki Saito, Takanori Kawano, Tadashi Nakaya, Toshiharu Suzuki*
Proteolysis of type-I membrane proteins and its signaling role in cells
27th Annual Meeting of Japan Molecular Biology Society
December 10, 2004 [W3E-7] *Invited Lecturer. JAPAN

14. Naoko Kato, Yoichi Araki, Akio Sumioka, Kazuo Yamamoto, Tadashi Nakaya, Toshiharu Suzuki
Cleavage of Alcadeins and its function
27th Annual Meeting of Japan Molecular Biology Society
December 9, 2004 [2PB-149] JAPAN

13. Naomi Miyagi, Yoichi Araki, Kazuo Yamamoto, Nasaki Mishimura, Miho Higuchi, Tadashi Nakaya,Toshiharu Suzuki
Metabolism of Alcadein, and potential marker for Alzheimerfs Disease
27th Annual Meeting of Japan Molecular Biology Society
December 9, 2004 [2PB-148] JAPAN

12. Chie Mizumaru, Tomohiro Yoshida, Yoichi Araki, Tadashi Nakaya, Toshiharu Suzuki
Charactalization of Alcadein-interacting proteins
77th Annual Meeting of Japan Biochemical Society
October 15, 2004 [3P-626] JAPAN

11. Megumi Sakuma, Yoichi Araki, Toshiharu Suzuki
Interaction of APP and Alcadein with X11L at the differnt Regions of PI/PTB domain
77th Annual Meeting of Japan Biochemical Society
October 15, 2004 [3P-625] JAPAN

10.Toshiharu Suzuki*, Yoichi Araki, Naomi Miyagi, Naoko Kato, Megumi Sakuma, Chie Mizumaru, Tadashi Nakaya
Regulation of APP metabolism and function by X11L and Alcadein
Neuro2004 (27th Annual meeting of Jpapan society for Neuroscience)
September 22, 2004 [SY15-02] *Invited Lecturer. JAPAN

9. Yoichi Araki, Naomi Miyagi, Naoko Kato, Tomohiro Yoshida, Toshiharu Suzuki
Alcadein is a novel substrate for Presenilin/gamma-secretase
26th Annual Meeting of Japan Biochemical Society
December 13, 2003 [4PC-053] Kobe, JAPAN

8. Tomohiro Yoshida, Yoichi Araki, Akio Sumioka, Hidenori Taru, Toshiharu Suzuki
AlcBP, novel Alcadein interacting protein affects metabolism of APP and Alcadein
26th Anneal Meeting of Japan Molecular Biology Society
December 13, 2003 [4PC-048] Kobe, JAPAN

7. Tomohiro Yoshida*, Yoichi Araki, Akio Sumioka, Hidenori Taru, Toshiharu Suzuki
Suprresion of beta-amyloid generation by novel membrane protein interacting with amyloid precursor protein and Alcadein
76th Annual Meeting of Japan Biochemical Society
October 16, 2003 [2P-522] *Lecturer. Yokohama, JAPAN

6. Yoichi Araki*, Susumu Tomita, Naomi Miyagi, Haruyasu Yamaguchi, Yutaka Kirino, Toshiharu Suzuki
Novel membrane proteins, Alcadein, enhances the X11L-mediated stabilization of APP metabolism and X11L-mediated supression of Abeta-generation
76th Anneal Meeting of Japan Biochemical Society
October 16, 2003 [2P-508] *Lecturer. Yokohama, JAPAN

5. Toshiharu Suzuki*, Yoichi Araki, Akio Sumioka,Tomohiro Yoshida, Eiko Kawaguchi, Mariko Shida, Hidenori Taru, Tadashi Nakaya
Regulation of the production and possible function of CTFgamma, a carboxy-terminal fragment of amyloid precursor protein cleaved by gamma-secretase
76th Anneal meeting of Japan Biochemical Society
October 16, 2003 [2S19-5] *Invited Lecturer. Yokohama, JAPAN

4. Yoichi Araki, Toshiharu Suzuki
Coupling of APP with novel membrane proteins through neuron-specific adaptor protein, X11-like
26th Anneal meeting of Japan Society for Neuroscience
July 23, 2003 [P2-P-166] JAPAN

3. Yoichi Araki, Susumu Tomita, Tomohiro Yoshida, Yasunori Shigenaga, Haruyasu Yamaguchi, Toshiharu Suzuki
Ecodomain shedding of Alcadein which interacts with X11-like
25th Annual meeting of Japan Molecular Biology Society
Decenmer 11, 2002 [1P-1344] Yokohama, JAPAN

2. Toshiharu Suzuki*, Yoichi Araki, Haruyasu Yamaguchi
Gene groups encoding novel cadherin molecule and its relationships of Neurodegenerative disorders
25th Anneal meeting of Japan Socity for Neuroscience
July 7, 2002 [S10-5]  *Invited Lecturer. Tokyo, JAPAN

1. Yoichi Araki, Susumu Tomita, Toshiharu Suzuki
Novel APP sorting pathway mediated by X11-like
24th Annual Meeting of Japan Moclecular Biology Society
December 12, 2001 [4P-582] Yokohama, JAPAN
*-oral or symposium  presenter

**Patents (International)
1. Toshiharu Suzuki, Yoichi Araki, Naomi Miyagi, Haruyasu Yamaguchi, Masaki Nishimura, Kazuo Yamamoto
MARKER PEPTIDE FOR ALZHEIMERfS DISEASE
(International) Patent No. WO/2005/044847 [Abstract]

**Patents (Domestic: Japan)
2. Toshiharu Suzuki, Yoichi Araki, Naomi Miyagi, Naoko Kato
Marker peptide for Alzheimerfs Disease
(Japan) Patent No. 2003-375363 (2003)

1. Toshiharu Suzuki, Yoichi Araki, Susumu Tomita
Novel Screening method for inhibitor of Abeta generating enzymes
(Japan) Patent No. 2001-367100 (2001)

Awards and Fellowships
6. 2005 Annual award of Japan Dementia Society  (To Toshiharu Suzki, Yoichi Araki, Eiko Kawaguchi, Katsuya Urakami, Masaki Nishimura, Sayaka Fujishige, Takao Ishikawa, Tadashi Nakaya , for hDiscovering beta-Alc alpha as potential marker for Alzheimerfs Diseaseh )
5. April. 2005- Mar. 2008 JSPS Fellowship for young scientists (To Yoichi Araki yBiology-#8772z)
4. April 2000- Mar 2005 Japan Student Service Organization Fellowship for Ph.D. course Students(To Yoichi Araki)
3. July 2006.7 10th International Conference of Alzheimerfs Disease Travel fellowship (To Yoichi Araki)
2. July 2004.7 9th International Conference of Alzheimerfs Disease Travel fellowship (To Yoichi Araki)
1. July 2002 8th International Conference of Alzheimerfs Disease Travel fellowship (To Yoichi Araki)

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